Expression site silencing and life-cycle progression appear normal in Argonaute1-deficient Trypanosoma brucei.
نویسندگان
چکیده
Christian J. Janzen a,1, Frederick van Deursen b,1, Huafang Shi c, George A.M. Cross a, Keith R. Matthews b, Elisabetta Ullu c,d,∗ a Laboratory of Molecular Parasitology, The Rockefeller University, Box 185, 1230 York Avenue, New York, NY 10021-6399, USA b Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, West Mains Road, Edinburgh, EH9 3JT, UK c Department of Internal Medicine, Yale Medical School, BCMM 136D, 295 Congress Avenue, Box 9812, New Haven, CT, 06536-8012, USA d Department of Cell Biology, Yale Medical School, BCMM 136D, 295 Congress Avenue, Box 9812, New Haven, CT, 06536-8012, USA
منابع مشابه
Expression site attenuation mechanistically links antigenic variation and development in Trypanosoma brucei
We have discovered a new mechanism of monoallelic gene expression that links antigenic variation, cell cycle, and development in the model parasite Trypanosoma brucei. African trypanosomes possess hundreds of variant surface glycoprotein (VSG) genes, but only one is expressed from a telomeric expression site (ES) at any given time. We found that the expression of a second VSG alone is sufficien...
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Expression of dsRNA complementary to small nucleolar RNAs (snoRNAs) in Trypanosoma brucei results in snoRNA silencing, termed snoRNAi. Here, we demonstrate that snoRNAi requires the nuclear TbDCL2 protein, but not TbDCL1, which is involved in RNA interference (RNAi) in the cytoplasm. snoRNAi depends on Argonaute1 (Slicer), and on TbDCL2, suggesting that snoRNA dicing and slicing takes place in ...
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ورودعنوان ژورنال:
- Molecular and biochemical parasitology
دوره 149 1 شماره
صفحات -
تاریخ انتشار 2006